中国兽医科学2012，Vol.42Issue(11)：1133-1138,6.
羊口疮病毒B2L蛋白二级结构及其细胞表位的预测
Prediction of secondary structure and cell epitopes of B2L protein of orf virus
摘要
Abstract
The secondary structure of B2L protein of orf virus(ORFV) was predicted using the network platform SOPMA server and DNAStar software. The B cell epitopes of ORFV B2L protein were predicted by comprehensive utilization of the DNAStar software, secondary structure prediction and ABCpred program. The cytotoxic T cell and T helper epitopes of ORFV B2L protein were predicted using the artificial neural network(ANNA) and the online program. The results showed that ORFV B2L protein was composed with a-helixes(30.42%) ,random coils(39.68%) ,β-sheet(24.34%) and β-turn(5.56%). And the ORFV B2L protein had 6 B cell epitopes, 3 cytotoxic T cell epitopes and 2 T helper epitopes. The result provided theoretical foundations for the establishment of ORFV diagnostic methods,preparation of monoclonal antibody and development of epitope vaccine against ORFV.关键词
羊口疮病毒/B2L基因/二级结构/B细胞表位/T细胞表位/预测Key words
orf virus/B2L gene/secondary structure/B cell epitope/T cell epitope/prediction分类
农业科技引用本文复制引用
王光祥,尚佑军,吕占禄,田宏,张克山,刘湘涛..羊口疮病毒B2L蛋白二级结构及其细胞表位的预测[J].中国兽医科学,2012,42(11):1133-1138,6.基金项目
国家现代肉羊产业技术体系项目 （）
“十一五”国家科技支撑计划项目 （）
