中国兽医科学2012，Vol.42Issue(11)：1168-1173,6.
Flotillin-1缺失突变体基因酵母双杂交诱饵载体的构建与鉴定
Construction and identification of flotillin-1 deletion mutant bait vector in yeast two-hybrid system and detection of its self-activation and toxic effect
摘要
Abstract
To construct flotillin-1 bait vector pGBKT7-X for the screening oi priori protein interaction molecular area,the flotillin-1 gene fragments were amplified by RT-PCR from bovine brain tissue, and cloned into the the pGBKT7 vector. The recombinant vectors were transformed into AH109,with the self-activation and toxic action of pGBKT7-X tested. Results indicated that parts of pG- BKT7-X were successfully constructed and proven to be no self-activation and no toxic to the host yeast. Two of pGBKT7-X proven to be self-activation and no toxic to the host yeast. The parts of pGBKT7-X could be used as bait to screen the protein molecular area interacting with prion protein in the yeast two-hybrid system.关键词
疯牛病/Flotillin-1蛋白/酵母双杂交/诱饵载体/自激活作用Key words
bovine spongiform encephalopathy/Flotillin-1 protein/yeast two-hybrid/bait vector/selfactivation分类
农业科技引用本文复制引用
马俊俊,刘永生,丁耀忠,周建华,高宗良,陈豪泰,马丽娜,张杰..Flotillin-1缺失突变体基因酵母双杂交诱饵载体的构建与鉴定[J].中国兽医科学,2012,42(11):1168-1173,6.基金项目
国家自然科学基金资助项目 （）
