中国兽医科学2012，Vol.42Issue(11)：1179-1183,5.
稳定表达猴源波形蛋白的BHK-21细胞系的建立
Establishment of a stable BHK-21 cell line expressing vimentin of Marc-145 cell
摘要
Abstract
In order to establish a stable BHK-21 cell line expressing vimentin of Marc-145 cell,the vi- mentin gene was amplified by RT-PCR from Marc-145 cells and inserted into pcDNA3.1/V5-His A plasmid together with EmGFP gene and IRES to construct recombinant plasmid pcDNA3. 1-GIV. The pcD- NA3.1-GIV plasmid was transfected into BHK-21 cell to establish stably expressing vimentin with the presence of G418 and then subcloned. Western-blot and RT-PCR results indicated that the BHK-21-GIV cells could express exogenous vimentin,which demonstrated that a cell line stably expressing vimentin was obtained, and designated as BHK-21-GIV. This study provided a useful tool for vimentin studying and provided evidence on the relationship between vimentin and porcine reproductive and respiratory syndrome virus.关键词
波形蛋白/稳定细胞系/BHK-21细胞/猪繁殖与呼吸综合征病毒Key words
vimentin/stable cell line/BHK-21 cell/porcine reproductive and respiratory syndrome virus分类
农业科技引用本文复制引用
马晓春,高继明,孔宁,高丽丽,王彤彤,肖一红,周恩民..稳定表达猴源波形蛋白的BHK-21细胞系的建立[J].中国兽医科学,2012,42(11):1179-1183,5.基金项目
国家自然科学基金资助项目 （）
