中国实用内科杂志2012，Vol.32Issue(10)：775-778,4.
尿毒症维持性血液透析近期胸腺输出功能变化研究
Measurement of recent thymic output function in uremia patients who underwent maintenance hemodialysis
摘要
Abstract
Objective To determine the recent thymic output of naive T cells in patients with uremia who underwent maintenance hemodialysis ( MHD). Methods Fifteen consecutive patients with uremia were recruited from Affiliated Oncology Hospital of Zhengzhou University between October 2010 and October 2011 and were subjected to detection of T cell receptor excision DNA circles (TRECs) in peripheral blood mononuclear cells (PBMCs) prior to and after MHD via real-time poly-merase chain reaction ( TaqMan). And the level of TRECs was calculated based on the positive rate of CD3 + T cells in peripheral blood. The peripheral blood drawn from 10 age-matched healthy individuals served as normal control. Results Prior to initial dialysis, 15 patients who underwent MHD were found to have considerably lower level of TREC [ ( 0. 48 ± 0. 39) copies/1000 PBMCs and ( 1. 03 ± 0. 57 ) copies/1000 CD3 + T cells; P - 0. 0014 and 0. 0067, respectively ] as compared with normal controls. Higher between-subject variation in peripheral blood TREC [ (0. 41 ±0. 36) copies/1000 PBMCs and (1. 33 ±1.46) copies/1000 CD3 + T cells;P =0. 0008 and 0. 019 Respectively] was shown at months 2 to 6 after MHD yet these levels remained remarkably lower than that of normal controls. The difference with pre-treatment level did not yield statistical difference ( P > 0. 05 ) . Conclusion Patients who underwent MHD are featured by markedly impaired recent thymic output naive T cell immunity prior to initial dialysis and at months 2 to 6 thereafter. Low recent thymic output function may contribute to cellular immunity defect.关键词
维持性血液透析/胸腺近期输出功能/免疫功能/T细胞受体重排删除环Key words
maintenance hemodialysis / recent thymic output function/ immune function/ T cell receptor rearrangement excision circles分类
医药卫生引用本文复制引用
李永新,尹青松,汪小娇,李倩玉,魏旭东..尿毒症维持性血液透析近期胸腺输出功能变化研究[J].中国实用内科杂志,2012,32(10):775-778,4.基金项目
国家自然科学基金(81000921) （81000921）
