中国畜牧兽医2012，Vol.39Issue(10)：7-10,4.
非洲猪瘟病毒VP73蛋白主要抗原表位区的原核表达及多克隆抗体的制备
Prokaryotic Expression of Major Antigenic Epitope Region of African Swine Fever Virus VP73 Protein and Preparation of Polyclonal Antibody
摘要
Abstract
Constructed recombinant prokaryotic expression vector pET32a-VP73 was transformed into E, colt BL21, major antigenic epitope region of VP73 protein was induced stably and efficiently by IPTG, the result of SDS-PAGE showed, when final concentration of IPTG was 1. 0 mmol/L, and pET32a-VP73 was induced 5 h, expression of major antigenic epitope region of VP73 protein was highest, the expressed protein was fusion protein, molecular weight was approximately 42 ku. The protein was identified as major antigenic epitope region of VP73 protein by SDS-PAGE and Western blotting. The purified major antigenic epitope region of VP73 protein was injected to New Zealand rabbits, serum was collected before and after injection. Antibody titer was determined by ELISA. Specificity of this protein was determined by ELISA whose primary antibody were ASF positive serum, rabbits serum before and after injection and positive serum of CSF, PRRS, pig pseudorabies. The result showed the titer of antiserum reached up to 1 : 1024000, and gave rise to reactions with VP73 major antigenic region protein. The polyclonal antibody built the foundation for immunology research of VP73 and serological diagnosis of African swine fever.关键词
非洲猪瘟/VP73/原核表达/多克隆抗体Key words
African swine fever/ VP73/ prokaryotic expression/ polyclonal antibody分类
生物科学引用本文复制引用
李洪利,王志亮,王君玮,张维,吴晓东,赵永刚,李慧,李娟,包静月,曹金山..非洲猪瘟病毒VP73蛋白主要抗原表位区的原核表达及多克隆抗体的制备[J].中国畜牧兽医,2012,39(10):7-10,4.基金项目
国家公益性行业(农业)科研专项经费(200903037). （农业）
