中国畜牧兽医2012，Vol.39Issue(10)：49-52,4.
2株猪繁殖与呼吸综合征病毒全基因序列分析
Sequence Analysis of Complete Genome of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
Ten gene fragments contains two strains of porcine reproductive and respiratory syndrome virus which were designated ZS-GD strain and ZH-GD strain complete genome were amplified respectively by RT-PCR, and cloned into pMD18-T vector for sequencing. Phylogenetic analysis of two strains of PRRSV complete genome sequence by bioinformatics software showed that the complete genome of ZS-GD and ZH-GD were 15320 bp, the genome of ZS-GD strain and ZH-GD strain shared 88. 1% to 97. 8% and 88. 4% to 98. 0% of nucleotide homology with American type strain, respectively; shared 60. 6% and 60.4% of nucleotide homology with Europe type strain(Lelystad virus). There were 30aa depletion in Nsp2 protein of two stains. The results showed that these two strains were depletion and variant strain of American type.关键词
PRRSV/基因组/序列测定/分析Key words
PRRSV/ genome/ sequencing/ analysis分类
生物科学引用本文复制引用
张民泽,闫明菲,谢杰雄,黄震,曹宗喜,吴欣伟,郭泗虎,张桂红..2株猪繁殖与呼吸综合征病毒全基因序列分析[J].中国畜牧兽医,2012,39(10):49-52,4.基金项目
现代农业产业技术体系建设专项资金资助(CARS-36). （CARS-36）
