中国畜牧兽医2012，Vol.39Issue(10)：73-75,3.
牛疱疹病毒Ⅰ型gB、gE基因的克隆及其杆状病毒表达载体的构建
Cloning of gB,gE Gene of Bovine Herpesvirus-1 and Construction of its Baculovirus Expression Vector
摘要
Abstract
The gB and gE gene of bovine herpesvirus-1 Bartha Nu/67 strain were amplified by PCR. The gB and gE fragment were cloned into pGEM-T-easy vector. The gB and gE gene were subcloned into baculovirus transfer vector and the re-combinant Baculovirus vector pFBHgB, pFBHgE were constructed successfully. Then they were transferred into E.coli DHlOBac and cultured in LB plate. The white bacterial colonies were positive recombinant bacmid named as BacmidgB, Bac-midgE. Thus the result mentioned above laid down theoretical and practical foundations for the expression of gB and gE gene in insect cells.关键词
牛疱疹病毒Ⅰ型/gB、gE基因/杆状病毒表达系统/载体构建Key words
bovine herpesvirus-1 (BHV-1)/ gB, gE gene/ baculovirus expression vector system/ vector.construction分类
生物科学引用本文复制引用
吴春涛,李艳梅..牛疱疹病毒Ⅰ型gB、gE基因的克隆及其杆状病毒表达载体的构建[J].中国畜牧兽医,2012,39(10):73-75,3.基金项目
东营职业学院院级课题(2010004). （2010004）
