中国畜牧兽医2012，Vol.39Issue(10)：87-90,4.
禽流感病毒WUDI-H9N2株的分离鉴定及HA基因的分子特征研究
Isolated and Identified of Avian Influenza Virus WUDI-H9N2 in China and Molecular Characteristics Research of HA Gene
谢银禄 1张玉 2刁富花2
作者信息
- 1. 青海省海西州格尔木市畜牧兽医站,青海格尔木816000
- 2. 青海省大通县后子河兽医站,青海大通810105
- 折叠
摘要
Abstract
An isolate of avian influenza virus, designated WUDI-H9N2, was isolated from Shandong area of China in Au-guste 2011 and identified for pathogenicity to chicken embryo by hemagglutination test, hemagglutination inhibition test and RT-PCR. A pair of primers was designed to amplify the HA strain based on the reported gene sequences in GenBank by Oligo 6. 0 and for WUDI-H9N2 HA gene amplifying and sequence analysis. The separated AIV H9 strain and other serotype were respectively analysed by nucleotide and amino acid phylogeny tree. The results of sequencing showed that the HA gene was 1708 bp in legth, no nucleotide insertion and deletion, the cDNA contains whole open reading frame(ORF) of HA gene was 1683 bp and encoded a 560 amino acid protein. Compared with reference strains, the nucleotide sequence similarity of HA was 92. 9% to 98. 1%. On the basis of phylogenetic analysis of HA gene, we concluded that the HA shared 98. 1% similarity of structure protein encoding sequence with A/chicken/China/AH-10-012010(H9N2) strain.关键词
禽流感病毒/HA基因序列/开放阅读框/遗传进化分析Key words
avian influenza virus/ HA gene sequence/open reading frame/ phylogenetic analysis
分类
生物科学引用本文复制引用
谢银禄,张玉,刁富花..禽流感病毒WUDI-H9N2株的分离鉴定及HA基因的分子特征研究[J].中国畜牧兽医,2012,39(10):87-90,4.
