中国畜牧兽医2012，Vol.39Issue(10)：91-94,4.
猪γ-干扰素全基因克隆及原核表达
Cloning and Prokaryotic Expression of Interferon Gamma Full Gene in Pig
马超英1
作者信息
- 1. 青海省海西州乌兰县铜普兽医站,青海乌兰817000
- 折叠
摘要
Abstract
To amplify pig interferon-y(pIFN-y) cDNA from ConA-activated peripheral-blood lymphocyte by RT-PCR, then was cloned into pMD18T and sequenced, and recombined with pET32a( + ) vector, the target protein HisX6 IFN-γ was induced by IPTG;the sequence analysis showed that the nucleotide sequences of the IFN-γ in the study shared 98. 6% to 100. 0% homology with that of pig published in GenBank, and the identity of amino acid was 96. 4% to 99. 4%. This recombinant protein , a molecular weight of about 27 ku and mainly presenting in inclusion bodies, was approved to have immunological activity by Western blotting analysis and SDS-PAGE. It laid a foundation for further research of IFN-γ.关键词
基因克隆/原核表达/IFN-γ基因/猪Key words
gene cloning/prokaryotic expression/interferon-γ gene/pig分类
生物科学引用本文复制引用
马超英..猪γ-干扰素全基因克隆及原核表达[J].中国畜牧兽医,2012,39(10):91-94,4.
