中国畜牧兽医2012，Vol.39Issue(10)：229-231,3.
猪伪狂犬病病毒北京株的分离鉴定
The Isolation and Identification of the Beijing Strain of Pig Pseudorabies Virus
张亮 1王文泉 2张坦 1郑杰 2张发明 1张红 2孙丰廷 2杨国良 2陈玲 2张洪2
作者信息
- 1. 河南农业大学牧医工程学院,河南郑州450002
- 2. 北京华都诗华生物制品有限公司,北京102600
- 折叠
摘要
Abstract
One virus strain was isolated from the samples of brain and internal organs of a death piglet from a hogpen in Beijing. ST cell line shrunk, aggregated and detached from the culture system in 24 h approximately. The isolate could be neutralized by PRV standard positive serum. Rabbits were injected with the isolate and the typical clinical syndrome could be observed, such as extra tickle. According to the gD gene sequences of PRV in GenBank, a pair of primers was designed, a specific fragment was amplified by PCR, the results of sequencing analysis showed that the amplified fragment was the gene sequence of the PRV. All of the evidences indicated that the isolated virus was pseudorabies virus, and was named Beijing strain in accordance with separate location.关键词
猪/伪狂犬病病毒/分离/鉴定Key words
swine/ pseudorabies virus/ isolation/ identification
分类
农业科技引用本文复制引用
张亮,王文泉,张坦,郑杰,张发明,张红,孙丰廷,杨国良,陈玲,张洪..猪伪狂犬病病毒北京株的分离鉴定[J].中国畜牧兽医,2012,39(10):229-231,3.
