中国畜牧杂志2012，Vol.48Issue(21)：28-31,4.
鸡胚胎干细胞的分离及其嵌合体制备条件探索
Isolation of Chicken Embryonic Stem Cell and Exploring the Preparation Conditions of Chicken Chimeric
摘要
Abstract
Chicken embryonic stem cells (ESCs) were separated and cultured in vitro. Alkaline phosphatase activity (AKP) and SSEA-1 staining was conducted to detected ESCs. Then, chicken ESCs were transfected with linearized plasmid pEGFP-Nl. The receptor eggs were drilled a window at the lateral shell of egg, then, the ESCs transfected with pEGFP-Nl was injected into chick embryo cavity so as to make chimeric chicken. The chimeric chicken was detected for both of blood and different tissue by PCR. The results showed five chimeric chicken were obtained in this study and found that four of them expressed EGFP gene in some organs, only two of them expressed EGFP gene in the gonad, indicating the chimeric chicken could be obtained through chick embryo cavity micro-injection by drilling a window at the lateral shell of egg.关键词
鸡胚胎干细胞/分离培养/显微注射/嵌合体鸡Key words
chicken embryonic stem cells/ separation and cultivation/ micro-injection/ chimeric chicken分类
农业科技引用本文复制引用
张亚妮,杨海燕,施青青,张振韬,郑蒙蒙,王丹,李碧春..鸡胚胎干细胞的分离及其嵌合体制备条件探索[J].中国畜牧杂志,2012,48(21):28-31,4.基金项目
高等学校博士学科点专项科研基金资助课题(20103250110006) （20103250110006）
江苏省"六大人才高峰" （）
中国博士后基金(2012M11806) （2012M11806）
