中国药房2012，Vol.23Issue(35)：3316-3319,4.DOI:10.6039/j.issn.1001-0408.2012.35.18
UPLC法测定藤茶中二氢杨梅素与杨梅素的含量及其热稳定性
Content Determination of Dihydromyricetin and Myricetin in Leaves of Ampelopsis grossedentata by UPLC and Investigation of Their Thermal Stability
摘要
Abstract
OBJECTIVE: To develop a method for the content determination of dihydromyricetin (DMY) and myricetin (MY) in leaves of Ampelopsis grossedentata, and to investigate the stability of DMY and MY in leaves of A grossedentata. METHODS: UPLC method was adopted. The separation was performed on Dionex Acclaim (R)RSLC PA2(30 mm×2.1 mm, 2.2 μm) column with 0.1% phorsphoric acid-acetonitrile as mobile phase (78:22, V/V) at flow rate of 0.4 mL·min-1. The detection wavelength of DMY was set at 290 nm (0～2 min), and that of MY was set at 373 nm (2～4 min). The changes of DMY and MY content were detected by hot reflux extraction. RESULTS: The linear range was 200.0～1 600.0 ng(r=0.999 8) for DMY and 5.0～56.2 ng(r=0.999 7) for MY with average recovery of 98.74% (RSD=1.90% , n=9) and 99.78% (RSD=1.37% , n=9). The content of DMY was 0.53%～35.01% and that of 0～0.62% , and total content of them was 0.53%～35.30%. DMY had poor thermal stability, so it was not recommended to use hot reflux for extraction. CONCLUSION: The method is fast, accurate and helpful for the quality control of leaves of A. grossedentata.关键词
超高效液相色谱法/藤茶/二氢杨梅素/杨梅素/含量测定/热稳定性Key words
UPLC/ Leaves of Ampelopsis grossedentata/ Dihydromyricetin/ Myricetin/ Content determination/ Thermal stability分类
医药卫生引用本文复制引用
樊兰兰,何丽丽,韦玮,曹芬,张亚洲,缪剑华..UPLC法测定藤茶中二氢杨梅素与杨梅素的含量及其热稳定性[J].中国药房,2012,23(35):3316-3319,4.基金项目
广西科学研究与技术开发计划项目(桂科攻0718006-3C) （桂科攻0718006-3C）
