中国药房2012，Vol.23Issue(35)：3323-3325,3.DOI:10.6039/j.issn.1001-0408.2012.35.21
秦巴山区不同产地娑罗子中七叶皂苷的含量比较
Comparison of the Contents of Escin in Aesculi Semen from Different Origins in Qinba Mountain Area
摘要
Abstract
OBJECTIVE: To compare the contents of escin in Aesculi Semen from different origins in Qinba mountain area. METHODS:HPLC method was adopted. The determination was performed on Hypersil ODS C18(100 mm×4.6 mm,5 urn) column with mobile phase consisted of acetonitrile-water-0.02% phosphoric acid (35:65, V/V) at flow rate of 1 mL·min-1. The detection wavelength was set at 220 nm and the column temperature was 20 ℃. RESULTS: The content of escin A (＞0.7%) was higher than that of escin B in all samples from 10 different producing areas in accordance with pharmacopeia (2010 edition). The contents of escin A and escin B from Lueyang county were 28.46 mg·g-1 and 19.77 mg·g-1 and were the highest, followed by samples from Kangxian and Liuba, Ningqiang, Mianxian, Ningshan, Zhenping, and the samples from Pingli, Xixiang and Yangxian were lower than them. The range of escin A content from 10 different producing areas was 1.16% ～2.85% , the range of escin B content was 0.89%～1.98% , and their averages were 1.92% and 1.43%. The ratios of escin A and escin B were 1.14～1.45. CONCLUSION: The contents and compositions of escins in Aesculi Semen from different origins in Qinba mountain area are significant. The influence of provenance, ecological environment and other factors to the accumulation of effective components in Aesculi Semen is obviously. To select material habitat is very important for stability guarantee of Aesculi Semen.关键词
娑罗子/七叶皂苷/秦巴山区/产地/高效液相色谱法Key words
Aesculi Semen/ Escin/ Qinba mountain area/ Producing area/ HPLC分类
医药卫生引用本文复制引用
张辰露,吴三桥,韩豪,周建军,缑慧君..秦巴山区不同产地娑罗子中七叶皂苷的含量比较[J].中国药房,2012,23(35):3323-3325,3.基金项目
陕西省教育厅科学研究项目(12JK0841) （12JK0841）
