摘要
Abstract
OBJECTIVE: To establish a method for simultaneous determination of notoginsenoside R1, ginsenoside Rg1, ginsen-oside Rbi, asperosaponin Ⅵ, psoralen and isopsoralen in Gukang capsules. METHODS: LC-MS method was adopted. The separation was performed on Agilent Zorbax SB-C18(150 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-water (gradient elution). The detection time was 30 min. The quantitative analysis of 6 components and internal standard was performed using mass spectrometry with electrospray ionization (ESI) interface in positive selective reaction monitoring (SRM) mode. RESULTS: The average recoveries of 6 components were 96.21% ～101.82% (r≥0.996 0). The variations of RSD ranged from 2.23% to 4.07% (n=6). The contents of 6 components in Gukang capsules, especially Asperosaponin Ⅵ, varied largely. CONCLUSION: The method is simple, sensitive and specific, and it is suitable for the quality evaluation of Gukang capsules; it is necessary to formulate strict content standard for the scientific quality evaluation of Gukang capsules.关键词
骨康胶囊/液相色谱-质谱联用技术/三七皂苷R1/人参皂苷Rg1/人参皂苷Rb1/川续断皂苷Ⅵ/补骨脂素/异补骨脂素/含量测定Key words
Gukang capsules/ LC-MS/ Notoginsenoside R1/ Ginsenoside Rg1/ Ginsenoside Rb1/ Asperosaponin Ⅵ/ Psoralen/Isopsoralen/ Content determination分类
医药卫生
