中国医科大学学报2012，Vol.41Issue(7)：588-590,3.
A型激酶锚定蛋白在小鼠卵母细胞减数分裂阻滞中的作用
Dynamic Anchoring of PKA via AKAPs is Essential for Meiotic Resumption of Mouse Oocytes
摘要
Abstract
Objective To explore the role of A-kinase anchoring proteins (AKAPs) in meiotic arrest of mouse oocytes. Methods Ht31 or Ht31-P peptide was microinjected into GV-stage mouse oocytes that were incubated in the presence of dbcAMP. The GVBD rates were counted and the phosphorylation status of Cdc2-Tyrl5 was determined by western blot. Results Ht31 stimulated oocyte maturation as indicated by GVBD and dephosphorylation of Cdc2-Tyrl5,which overcame the dbcAMP-induced arrest. In the control group,the inactive Ht31-P had no effect on GVBD,and Cdc2-Tyrl5 was phosphorylated. Conclusion PKA (protein kinase A) interaction with AKAPs is essential for the initial maintenance of meiotic arrest in mouse oocytes.关键词
卵母细胞/减数分裂阻滞/A型激酶锚定蛋白Key words
oocytes/meiotic arrest/A-kinase anchoring proteins分类
生物科学引用本文复制引用
王述森,张震,罗阳,张阳..A型激酶锚定蛋白在小鼠卵母细胞减数分裂阻滞中的作用[J].中国医科大学学报,2012,41(7):588-590,3.基金项目
国家自然科学基金青年基金资助项目(30800392) （30800392）
辽宁省教育厅高校科研计划(2008807) （2008807）
