中国医科大学学报2012，Vol.41Issue(7)：618-621,4.
β-榄香烯对血管内皮细胞成血管能力、细胞凋亡及MMP-2和MMP-9活性的影响
Effects of β-elemene on Angiogenesis, Apoptosis and MMP-2, MMP-9 Activities in Endothelial Cells
李悦 1杨向红 1刘云鹏2
作者信息
- 1. 中国医科大学附属盛京医院病理科,沈阳110004
- 2. 中国医科大学附属第一医院肿瘤科,沈阳110001
- 折叠
摘要
Abstract
Objective To investigate the effects of P-elemene on the vasculogenesis,apoptosis and the activity of MMP-2,MMP-9 in endothelial cells, and to explore the inhibiting effect of P-elemene on tumor angiogenesis. Methods ECV304 cells were cultured in vitro, and treated with P-elemene; the angiogenie ability of ECV304 cells was evaluated on Matrigel. Apoptosis were analyzed by using flow cytometry, and the activity of MMP-2 and MMP-9 in ECV304 cells were delected by -Zymography. Results Comparing with the control group,the tube formation from the P-elemene treated ECV304 cells on Matrigel notably decreased. With the increase of the dose and administration time,the apoptosis of ECV3O4 cells was significantly increased. P-elemene inhibited the activity of MMP-2 and MMP-9 in endothelial cell. Conclusion P-elemene can enhance apoptosis of the endothelial cells cultured in vitro, inhibit their angiogenie ability, reduce the number of tube formation of ECV304 cells on Matrigel,and inhibit the activity of MMP-2 and MMP-9.关键词
β-榄香烯/血管内皮细胞/细胞凋亡/成血管能力/基质金属蛋白酶2/基质金属蛋白酶9Key words
β-eleiiiene/vascular endothelial cell/apoptosis/angiogenesis/matrix metoproteinase-2/matrix metoproteinase-9分类
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李悦,杨向红,刘云鹏..β-榄香烯对血管内皮细胞成血管能力、细胞凋亡及MMP-2和MMP-9活性的影响[J].中国医科大学学报,2012,41(7):618-621,4.
