中国医科大学学报2012，Vol.41Issue(8)：682-685,4.
siRNA介导的新趋化因子VCC-1沉默对人SMMC7721肝癌细胞生长的影响
Effect of RNA Interference of New Chemokines VCC-1 on Proliferation of SMMC7721 Hepatocellar Carcinoma Cells
摘要
Abstract
Objective To study the effect of small interfering RNA (siRNA) mediated VCC-1 silencing on proliferation of hepatocellular carcinoma cell line SMMC7721. Methods Recombinant interfering plasmid was transfected into hepatocellular carcinoma cell line SMMC7721 by lipofectamineTM 2000. The efficiency of VCC-1 down regulation was assessed by Western blot. The effects of VCC-1 down regulation on the proliferation of SMMC7721 cells were determined by MTT assay and plate clone formation assay. Results Compared with the negative control and parental group,Western blot results demonstrated that the protein level of VCC-1 was significantly decreased (P < 0.01). Silencing of VCC-1 in SMMC7721 cells significantly suppress cellular proliferation rate and inhibit the ability of colony formation (P < 0.05). Conclusion Targeting of VCC-1 gene by siRNA can significantly inhibit the growth and proliferation in SMMC7721 cells.关键词
VCC-1/肝癌/SMMC7721细胞/siRNAKey words
VCC-1/ hepatocellular carcinoma/ SMMC7721 cell line/ siRNA分类
医药卫生引用本文复制引用
牟霞,陈瑶,王穗海,李明..siRNA介导的新趋化因子VCC-1沉默对人SMMC7721肝癌细胞生长的影响[J].中国医科大学学报,2012,41(8):682-685,4.基金项目
国家高技术研究发展计划(863)资助项目(2006AA02A311) （863）
