中国医科大学学报2012，Vol.41Issue(8)：696-699,4.
布洛芬对小鼠肺纤维化的保护作用
Experimental Study of Protective Effect of Ibuprofen on the Pulmonary Fibrosis in Mice
摘要
Abstract
Objective To study the protective effect of ibuprofen on bleomycin-induced pulmonary fibrosis. Methods The pulmonary fibrosis mouse model was established by intratracheal instillation of bleomycin. The experimental mice were divided into normal control group, model group,5 mg/kg brufen treated group, 10 mg/kg brufen treated group,and 20 mg/kg brufen treated group. Serum was collected to detect the content of NO by NO chemical method; lavage fluid was collected for determination of TNF-a and IL-6 levels by using ELISA; specimens of lung tissue were stained with HE to observe the morphological changes, and lung homogenates were prepared for determination of malondi-aldehyde (MDA);the expression levels TGF-β1 ,Smad2 and p-Smad2 in lung tissue were detected by RT-PCR and Western blot analysis. Results Compared with the normal control group, inflammatory cell infiltration, alveolar septal thickening occurred in lung tissue of pulmonary fibrosis model; meanwhile, NO content in serum and inflammatory cytokines TNF-a and IL-6 levels in bronchoalveolar lavage fluid were significantly increased,MDA content was also increased in the lung tissue of mice,the expressions of TGF-β1 and p-Smad2 were significantly increased;after ibuprofen administration,various biochemical indicators declined,and pulmonary fibrosis damage was mitigated. Conclusion Ibuprofen can alleviated bleomycin-induced pulmonary fibrosis,and the mechanism may be associated with lowered content of NO, TNF-α, IL-6 and other inflammatory cytokines content and regulation of TGF-β signaling pathway.关键词
布洛芬/博莱霉素/肺纤维化Key words
ibuprofen/ bleomycin/ pulmonary fibrosis分类
医药卫生引用本文复制引用
王春雷,张钰淼,张烨..布洛芬对小鼠肺纤维化的保护作用[J].中国医科大学学报,2012,41(8):696-699,4.基金项目
辽宁省教育厅高校科研计划(2010686) （2010686）
