中国医科大学学报2012，Vol.41Issue(8)：713-715,718,4.
肺孢子菌繁殖方式的进一步研究
Further Observation on Reproduction Mode of Pneumocystis from Dexamethasone-treated Rats
摘要
Abstract
Objective To study the reproduction mode of Pneumocystis from dexamethasone- treated Wistar rats. Methods Ten female Wistar rats were injected subcutaneously with 1 mg of dexamethasone acetate twice a week to establish the Pneumocystis pneumonia (PCP) model . The rats were sacrificed at 9 weeks after the initiation of treatment. The organisms of Pneumocystis (p.c) were obtained from the bronchoalveolar lavage fluid (BALF) of the ten rats with Pneumocystis pneumonia (PCP). The pellets of BALF were stained with Gomori methenamine silver stain (GMS) to ensure the presence of p.c. The reproduction of Pneumocystis organisms from bronchoalveolar lavage fluid (BALF) of rats was studied with scanning electron microscopy (SEM),and the reproduction of Pneumocystis organisms in rats' lungs was studied with the transmission electron microscopy (TEM). Results The PCP rat model was successfully established and the organisms were found on full of the visual fields. In addition to the observation of binary fission and the conjugation of the trophic forms from the BALF and the lung sections with SEM and TEM, the exogenous budding of the trophic form and the endogenous budding of the cyst were observed from the lung sections with TEM in present study. Conclusion Sexual and asexual reproduction coexist in the life cycle of Pneumocystis organisms. The trophic forms maybe reproduce themselves by binary fission,conjugation and exogenous budding,and the cysts by endogenous budding.关键词
肺孢子菌/二分裂增殖/接合生殖/外出芽生殖/内出芽生殖Key words
pneumocystis/ binary fission/ conjugation/ exogenous budding/ endogenous budding分类
医药卫生引用本文复制引用
邴玉艳,安春丽,刘月华,刘冬娟..肺孢子菌繁殖方式的进一步研究[J].中国医科大学学报,2012,41(8):713-715,718,4.基金项目
河北省卫生厅基金资助项目(20090578) （20090578）
