中国医科大学学报2012，Vol.41Issue(8)：719-722,4.
环孢素A联合脂多糖对牙周膜成纤维细胞生物学活性的影响
Effects of Cyclosporine A and Porphyromonas gingivalis-lipopolysaccharide on Biological Activity of in vitro Cultured Human Periodontal Ligament Fibroblasts
摘要
Abstract
Objective To evaluate the in vitro effects of cyclosporine A (CsA) and Porphyromonas gingivals-lipopolysaccharide(Pg-LPS) on the biological activity of human periodontal ligament fibroblasts (hPDLFs). Methods hPDLFs were incubated with CsA 100 ng/mL,Pg-LPS 100 ng/mL,CsA 100 ng/mL+Pg-LPS 100 ng/mL. Then the total protein was extracted and the concentration was assessed with Coomassie brilliant blue. Several features such as expression of bone morphogenetic protein-2 (BMP-2) and alkaline phosphatase(ALP) were detected by enzyme-linked immunosorbent assay (EL1SA). The formation of mineralized islets was assessed by Von kossa staining. Results CsA alone or plus LPS could significantly enhanced the total protein concentration, ALP, BMP-2 and mineralized islets of hPDLFs; LPS alone could significantly promote cells ALP expression, whereas has no significant effect on protein concentration, BMP-2 and mineralized islets of hPDLFs. Conclusion At certain concentrations, CsA could significantly enhance hPDLFs biological activity, synergistic effect was observed on enhancing cell differentiation when combined with LPS.关键词
环孢素A/脂多糖/牙周膜成纤维细胞Key words
cyclosporine A/ Porphyromonas gi'ngiValis-lipopolysaccharide/ periodontal ligament fibroblast分类
医药卫生引用本文复制引用
姚丽艳,黄美香,钟泉,郑瑜谦,李大兰,骆凯,闫福华..环孢素A联合脂多糖对牙周膜成纤维细胞生物学活性的影响[J].中国医科大学学报,2012,41(8):719-722,4.基金项目
福建省科技重点项目(2009Y0019) （2009Y0019）
福建医科大学临床医学重点学科建设项目(XK201111) （XK201111）
