中国医科大学学报2012，Vol.41Issue(8)：738-741,4.
实时定量聚合酶链反应与巢式聚合酶链反应在289例白血病融合基因检测中的比较
The Comparison of RT-PCR and Nested PCR in Detection of Fusion Genes in 289 Cases with Leukemia
摘要
Abstract
Objective To explore the differences of real-time quantitative polymerase chain reaction (RT-PCR) and nested polymerase chain reaction (nPCR) in the detection of fusion genes of BCR/ABL,PMWRARa and AML1/ET0 in leukemia. Methods RT-PCR and nPCR were applied for detection of the fusion genes BCR/ABL,PML/RARa and AML1/ET0 in 289 patients with leukemia,the results combined with cytomophology and clinical outcomes of patients were analyzed. Results The positive rates of fusion gene of BCR/ABL detected by RT-PCR and nPCR in 46 cases of newly diagnosed chronic myelocytic leukemia (CML) were 95.7% and 93.5% respectively (P = 0.997),while the ratios were 78.4% and 58.1% (P < 0.05) in 69 cases of complete remission (CR). By the same detections,the positive rates of fusion gene of PML/RARa in 32 cases of newly diagnosed acute promyelocytic leukemia (APL) were 93.8% and 90.6% respectively (P =0.996),whae the ratios were 12.3% and 7.0% (P<0.05) in 114 cases of CR. The positive rates of fusion gene of AML1/ETO in 12 cases of newly diagnosed acute myeloblastic leukemia M2 (ANLL-M2) were 50.0% and 50.0% respectively (P =1.0),while the ratios were 50.0% and 12.5% (P < 0.05) in 16 cases of CR. Conclusion RT-PCR is more sensitive and accurate than nPCR for detecting fusion genes of leukemia. It can improve the detection of minimal residual disease, and provide more effective molecular biological basis for diagnosis and treatment in leukemia.关键词
白血病/融合基因/实时定量聚合酶链反应/巢式聚合酶链反应Key words
leukemia/ fusion genes/ real-time quantitative polymerase chain reaction/ nested polymerase chain reaction分类
医药卫生引用本文复制引用
王玥,李艳,金晶纯,王亚柱,魏鑫..实时定量聚合酶链反应与巢式聚合酶链反应在289例白血病融合基因检测中的比较[J].中国医科大学学报,2012,41(8):738-741,4.基金项目
辽宁省自然科学基金资助项目(20082117) （20082117）
辽宁省教育厅高校科研计划(2008782) （2008782）
