中国医科大学学报2012，Vol.41Issue(9)：776-779,4.
肿瘤坏死因子α对急性肝功能衰竭小鼠中血脑屏障紧密连接的影响及机制
Impact and Mechanisms of Tumor Necrosis Factor-α on the Blood-brain Barrier Tight Junction in Acute Liver Failure Mice
摘要
Abstract
Objective To investigate the mechanism of tumor necrosis factor-a (TNF-α) in changing the permeability of the Hood-brain barrier (BBB) during acute liver failure (ALF). Methods ALF rat models were established by administering D-galactosamine (D-GalN) and TNF-α. We tried to investigate the BBB permeability with method of Evas blue (EB) staining and detect the BBB structure using electron microscopy. Expressions of TJ-associated protein occludin protein were determined by Western blot. Results Increased BBB permeability in ALF mice was found in ALF rats. Tight junction (TJ) disruption and EC shrinkage with increased vesicles and vacuoles were detected in the model. In addition,the expression of occludin was markedly reduced in the model. Conclusion Our results suggested that TNF-α plays a critical role in the development of brain edema in ALF rats. Increased BBB permeability might attribute to the disruption of TJs, and the reduction of occludin expression.关键词
急性肝功能衰竭/肿瘤坏死因子α/紧密连接Key words
acute liver failure/tumor necrosis factor-α/tight junction分类
医药卫生引用本文复制引用
韩家峰,吕飒,王文,刘沛..肿瘤坏死因子α对急性肝功能衰竭小鼠中血脑屏障紧密连接的影响及机制[J].中国医科大学学报,2012,41(9):776-779,4.基金项目
国家自然科学基金资助项目(97100252) （97100252）
