中国医科大学学报2012，Vol.41Issue(9)：788-790,794,4.
促红细胞生成素对缺血再灌注心肌HSP70及ERK1/2表达的影响
Effects of Erythropoietin on the Expression of ERK1/2 and HSP70 in Myocardial Ischemia-reperfusion Injury
摘要
Abstract
Objective To study the expression of HSP70,ERK 1/2 and their possible links to the effects of EPO preconditioning. Methods A rat model of myocardial I-R injury was established by ligating the left descending coronary artery for 30 min and then reperfusing for 2 hours. Totally 24 rats were randomly divided into 3 groups:sham operation group(ip saline 2 mL,24 hours before the operation,n = 8),control group(ip saline 2 mL,24 hours before the operation,n = 8),and EPO group(ip EPO,5 000 U/kg,diluted in 2 mL of saline solution,24 hours before the operation,n = 8). The sham-operated group was subjected to thoracotomy and passage of a silk ligature around the LCA without ligation. The Expression of HSP70 mRNA and ERK1/2 mRNA in the left ventricle were analyzed by RT-PCR,and the correlation between HSP70 mRNA and ERK1/2 mRNA expression was analyzed. Results HSP70 mRNA levels in the EPO preconditioning group (0.850+0066) were significantly higher than control group (0.355±0.043,P < 0.01) and sham-operated group (0.083+0.017,P < 0.01 ); ERK1/2 mRNA in the EPO group (1.150+0.125) were significantly higher than control group (0.576±0.116,P < 0.01) and sham-operated group(0.044±0.010,P <0.01); The correlation between HSP70 mRNA and ERK1/2 mRNA was significant(r =0.965,P < 0.01). Conclusion A single ip injection of 5 000 units/kg of EPO at 24 hours pre-ligation enhanced the expression of HSP70 and ERK1/2 in rat myocardium , and that the possible mechanism for the increasing of HSP70 is the increased expression of ERK1/2.关键词
促红细胞生成素/HSP70/ERK1/2/心肌缺血再灌注损伤Key words
erythropoietin/HSP70/ERK1/2/cardiac ischemia-reperfusion injury分类
医药卫生引用本文复制引用
王娜娜,王文生,刘鑫璐..促红细胞生成素对缺血再灌注心肌HSP70及ERK1/2表达的影响[J].中国医科大学学报,2012,41(9):788-790,794,4.基金项目
辽宁省自然科学基金资助项目(20092107) （20092107）
