中国医科大学学报2012，Vol.41Issue(10)：892-895,899,5.
氧化苦参碱对高果糖喂养诱导大鼠脂肪肝和肝脏内质网应激的干预作用
Effects of Oxymatrine on High-fructose-feeding-induced Fatty Liver and Endoplasmic Reticulum Stress in Rats
摘要
Abstract
Objective To observe the effects of oxymatrine intervention on high-fructose-feeding-iraiuced liver steatosis in rats. To analyze the effects of high -fructose-feeding on endoplasmic reticulum (ER) stress in rats liver and the intervention effects of oxymatrine. Methods Male Wistar rats were randomly divided into control group, high fructose group and oxymatrine group (oxymatrine intervention was started after 4 weeks of high fructose feeding,40 mg/kg). Rats were sacrificed after 16-weeks of high fructose feeding. Fasting blood glucose,fasting serum insulin were measured. HOMA index were calculated. Serum alanine aminotransferase (ALT),serum asparlate aminotransferase (AST) and liver triglyeeride content were determined. Gene expressions of GRP78 and CHOP in liver were detected. Protein levels of ER stress markers such as phosphorykted pancreatic ER kinase (p-PERK), phophorylated inositol-requiring enzyme 1 (p-IREl ) and activation transcription factor-6 (ATF-6) in liver tissues were detected. Results Compared with control group, FBG,FINS, HOMA index,serum ALT, serum AST and triglyeeride contents in liver tissues were significantly increased in high-fructose group (P < 0.01 ). Compared with high-fructose group,all above mentioned index were significantly lower in oxymatrine group(P < 0.01 ).The gene expression of GRP78 and CHOP and the protein expression of p-PERK,p-IREl and ATF-6 were significantly increased in high-fructose group compared with control group ( P < 0.01 ). While in oxymatrine group,both gene markers and protein markers of ER stress in liver were decreased ( P <0.01 ). Conclusion Long-term high-fructose-feeding can induce liver steatosis and liver cell injury in rats,and accompanied by liver cell ER stress. Oxymatrine treatment can improve high-fructose-feeding-induced fatty liver and liver cell injury,in which the underlying mechanism might be associated with the alleviation of ER stress through oxymatrine intervention.关键词
氧化苦参碱/甘油三酯/脂肪肝/内质网应激Key words
oxymatrine/triglyceride/fatty liver/endoplasmic reticulum stress分类
医药卫生引用本文复制引用
任路平,宋光耀,霍丽静,刘娜,陈树春..氧化苦参碱对高果糖喂养诱导大鼠脂肪肝和肝脏内质网应激的干预作用[J].中国医科大学学报,2012,41(10):892-895,899,5.基金项目
河北省科技厅国际合作项目(11396406-D) （11396406-D）
