中国医科大学学报2012，Vol.41Issue(10)：921-925,5.
齐墩果酸诱导Jurkat细胞凋亡及对ROS和细胞内Ca2+含量的影响
Oleanolic Acid Introduced Apoptosis in Jurkat Cells and Influence on the Contents of ROS and Intracellular Ca2+
摘要
Abstract
Objective To investigate the effect of oleanolic acid (OA) on proliferative inhibition and apoptotic induction in Jurkat cells, and explore the relationship between OA-induced apoptos's and the change of intracellular ROS and Ca2+ concentration. Methods The cell inhibitory rate was assessed with Mil assay. The apoptotic rate and the cycle phase of Jurkat cells were measured with Dow cytometry. ROS level was observed by DCFH-DA fluorescent quantitation, while intracellular Ca2+ concentration was measured using Fluo-3AM load method. Results The proliferation of Jurkat cells was inhibited by OA in a dose- and time-dependent manner. The apoptotic rates of Jurkat cells treated with OA (0,40,80 and 160 μmol/L) for 24 h were(7.36±0.40)%.( 19.80±1.59)%, (29.39±0.64)% and( 34.72+0.94)% and the cells of Go/G, phase were(54.26± 1.43)%, (85.83±0.91 )%, (91.18+1.32)% and(92.90±1.19)%. ROS and intracellular Ca2+ concentration of treatment with 80 u,mol/L and 160 μmol/L OA were higher than control( P < 0.05) and were positive correlated with apoptotic rates of Ju- rkat cells(r =0.95 and r =0.97). Conclusion The increase of ROS and intracellular Ca2+ might be involved in oleanlic acid-induced Jurkat cells apoptosis.关键词
齐墩果酸/Jurkat/细胞凋亡/细胞内活性氧/细胞内钙离子Key words
oleanolic acid/Jurkat/cell apoptosis/reactive oxygen species/intracelluar Ca2+分类
医药卫生引用本文复制引用
李旸,廖爱军,潘梦瑶,周蕾,徐淑梅,刘卓刚..齐墩果酸诱导Jurkat细胞凋亡及对ROS和细胞内Ca2+含量的影响[J].中国医科大学学报,2012,41(10):921-925,5.基金项目
辽宁省科学技术计划(2010225032) （2010225032）
