中国医科大学学报2012，Vol.41Issue(10)：926-929,4.
Smad3小干扰RNA在转化生长因子β1作用下对树突状细胞成熟及功能的影响
Effects of Smad3 SiRNA on Maturity and Function of Dentritic Cells with Transforming Growth Factor-β1
摘要
Abstract
Objective To investigate the effects of small interference RNA (siRNA) targeting Smad3 on the maturity and function of den-trictic cell (DC) with transforming growth faetor-β1 (TGF-β1). Methods The siRNA sequences targeting Smad3 gene were designed,and Uansfected to DC by RNAiMAX. The transfection efficiency was detected by RT-PCR. The experimental classification was performed as followed : group of Control-DC, group of TGF-β1-Control-DC, group of TGF-β1-Negative siRNA-Smad3 DC and group of TGF-prsiRNA-Smad3 DC. The maturity of DC was investigated by FCM. The level of IL-12 was determined by ELISA and the proliferation of allogenic T cell stimulated with DC was detected by CCK-8 assays. Results Expression of CD80, CD83, CD86 and HLA-DR and level of IL-12 in group of TGF-β1-siRNA-Smad3 DC was higher than both TGF-β1-Controt-DC group and TCF-P,-Negative siRNA-Smad3 DC group (P < 0.05). The group of TGF-prsiRNA-Smad3 DC had higher stimulation on the allogenic T cell proliferation. Conclusion Block Sniad3 expression in DC by siRNA can reverse the TGF-β1 inhibition on the maturity and immune function of DC.关键词
树突状细胞/转化生长因子β1/Smad 3/肿瘤疫苗Key words
dentritic cells/transforming growth factor-?/Smad 3/tumor vaccines分类
医药卫生引用本文复制引用
马楠,祁馨卉,崔慧霞,李妍,刘云鹏,姜又红..Smad3小干扰RNA在转化生长因子β1作用下对树突状细胞成熟及功能的影响[J].中国医科大学学报,2012,41(10):926-929,4.基金项目
辽宁省科学技术计划项目(2011415052-3) （2011415052-3）
