中国医科大学学报2012，Vol.41Issue(10)：934-936,3.
腹腔冲洗液BNIP3基因甲基化与胃癌患者腹膜微转移及疾病的相关性研究
Correlation Study between Gastric Cancer Peritoneal Micrometastases and BNIP3 Methylation in Peritoneal Lavage Fluid
摘要
Abstract
Objective To investigate whether gene methylation of BNIP3 in the peritoneal fluid(PF) predicts peritoneal micrometastases in gastric cancer patients. Methods Eighty pairs of adjacent normal gastric mucosa and corresponding primary cancer tissues were collected from surgery,and peritoneal lavage fluid was also obtained from these 80 patients. The gene methylation of BNIP3 were analyzed by quantitative methylation-specific polymerase chain reaction (MSP). Results Gene BNIP3 showed a significantly higher methylation level in primary cancer tissues than the adjacent normal gastric mucosa (P < 0.05). Conclusion BNIP3 methylation in peritoneal lavage fluid analyzed by MSP can be used for the prediction of peritoneal micrometastases in patients with gastric cancer and assessment of tbe disease progress.关键词
BNIP3/甲基化/胃癌/微转移/腹腔冲洗液Key words
BNIP3/methylation/gastric cancer/micrometastases/peritoneal lavage fluid分类
医药卫生引用本文复制引用
王银玲,王亿龙,王齐晖,李花,周立平,李富顺..腹腔冲洗液BNIP3基因甲基化与胃癌患者腹膜微转移及疾病的相关性研究[J].中国医科大学学报,2012,41(10):934-936,3.基金项目
辽宁省教育厅高校科研计划(L2010695) （L2010695）
