中国药理学通报2012，Vol.28Issue(11)：1527-1530,4.DOI:10.3969/j.issn.1001-1978.2012.11.011
环六缩酚肽抑制人前列腺癌细胞增殖活性及作用机制研究
Inhibotory effect of cyclic hexadepsipeptides on the protiferation activity of human PC-3 cells
摘要
Abstract
Aim To study the effeet induced by three cyclic hexadepsipeptides isolated from fungi and to explore its mechanism. Method Human PC-3 cells were cukured in vitro and then treated with cyclic hexadepsipept ides at the concentration of 10 μmol · L~1 . The effeet of three cyclic hexadepsipeptides on human PC-3 cells was measured by MTT assay. The apopLosis of the cells was evaluated by TUMEL assay. The protein expression of apopLosis in PC-3 cells was analyzed by WesLern blot assay. Results After three cyclic hexadepsipeptides ( 10 μmol ·L~1 ) LreaLment, Pullularin C showed significant activities against human PC-3 cells; Cell apopLosis of Pullularin C treated group was significantly higher than that in control group, and which also significantly up-regulated the Bax and caspase-3 protein expression compared with control group in PC-3 cells. Conclusions Pullularin C has a significanL inhibiLory effcct on the protiferation of human PC-3 cells. Besides, Pullularin C can also induce cells apoptosis through the mediation of Bax and caspase-3.关键词
环六缩酚肽/前列腺癌细胞/抑制活性/细胞凋亡/Bax/Caspase-3Key words
cyclic hexadepsipepLides/ human PC-3 cells/ anti-growth effect/ apoptosis/ Bax / caspase-3分类
医药卫生引用本文复制引用
倪晓辰,张爱莉,赵志红,史清文..环六缩酚肽抑制人前列腺癌细胞增殖活性及作用机制研究[J].中国药理学通报,2012,28(11):1527-1530,4.基金项目
国家自然科学基金资助项目(No 81072551) （No 81072551）
河北省自然科学基金资助项目(No C2010000489) （No C2010000489）
河北省科技支撑项目(No 11276103D-89) （No 11276103D-89）
