中国药理学通报2012，Vol.28Issue(11)：1535-1538,4.DOI:10.3969/j.issn.1001-1978.2012.11.013
大黄素对鼻咽癌CNE-1细胞放射增敏作用与细胞自噬关系的研究
Study of the relationship between the radiotherapy sensitization function of emodin and autophagy in the nasopharyngeal carcinoma CNE- 1 cells
摘要
Abstract
Aim To invesLigaLe vvheLher emodin induces autophagy in Lhe nasopharyngeal carcinoma CME-1 colls aftcr radiaLion combined with emodin and explore the relationship between the radiotherapy sensi-tization and autophagy-related gene mRMA expression. Methods Masopharyngeal carcinoma CME-1 cells were divided into control group, emodin alone group, radiation alone group and radiaLion combined with emodin group. After different treatmenLs, the ultrastructur-al changes of autophagosome were observed by trans-mission electron microscope( TEM ). The mRMA expressions of ULK.1 , GABARAPL1 , Beclinl and Bcl-2 were detected by Lhe Real-time PCR. Results Autophagosome or autophagolysosome was observed in all groups except in the control cells. Compared with the control group, the resuks of Real-time PCR showed that ULK1 mRMA expression in all the experimental groups increased( P <0. 05 ). The radiotherapy sensiti-zation group showed the highest( P <0. 01 ). However, Beclinl and Bcl-2 mRMA expression were down-regu-lated. GABARAPL1 expression had no remarkable changes among the experimental groups, exeept in radiotherapy sensitizaLion group where it was up-regulated ( P <0. 05 ). Conclusions Emodin induces autophagy in the nasopharyngeal carcinoma CME-1 cells after radiation combined with emodin. Autophagic cell death ( ACD ) may be one of the paths of the radiosensitiza-tion sensitization effect.关键词
鼻咽癌/大黄素/放射增敏/自噬/透射电镜/定量PCRKey words
nasopharyngeal carcinoma/ emodin/ radiotherapy sensitization/ autophagy/ transmission electron microscope( TEM )/ Real-time PCR分类
医药卫生引用本文复制引用
陈冬莲,侯华新,郑华,梁燕,黎丹戎..大黄素对鼻咽癌CNE-1细胞放射增敏作用与细胞自噬关系的研究[J].中国药理学通报,2012,28(11):1535-1538,4.基金项目
国家自然科学基金资助项目(No 81060270) （No 81060270）
广西医学科学实验中心开放基金资助项目(No KFJJ2011-25) （No KFJJ2011-25）
