中国药理学通报2012，Vol.28Issue(11)：1553-1556,4.DOI:10.3969/j.issn.1001-1978.2012.11.017
铁皮石斛多糖对RAW2647细胞分泌TNF-α的影响
Effects of polysaccharides from Dendrobium Officinale on the production of TNF-α by RAW264.7 cells
摘要
Abstract
Aim To sLudy die effects of polysaccharides from dendrobium officinale ( DOP ) on the pro-due don of TMF-a in RAW 264. 7 cells and its possible mechanism. Methods Cell proliferation was measured by MTT assay; Concentration of TMF-a in super-natants was determined by FLISA kit; Expression of TMF-α mRMA was measured by reverse transcription polymerase chain reaction ( RT-PCR ); Production of 1-κBα protein was detected by Western blot. Results DOP (20-160 mg o L-1 ) possessed the stimulation effect on macrophages proliferation without cytotoxic. The production of TMF-α was increased in a dose-dependent manner when pretreated with DOP. Compared with control group, expression of TMF-α mRMA was significantly up-regulated. DOP markedly decreased intracellular 1-κBα level as demonstrated by Western blot assay. Conclusion DOP induces TMF-α gene expression followed by TMF-α production in macropha-ges via decreasing intracellular 1-κBα level, activating transcription factor, MF-ΚB, suggesting that the immu-noregulation mechanism of DOP may be related to the induction of TMF-α production in macrophages.关键词
铁皮石斛多糖/RAW264.7细胞/细胞增殖/TNF-α/TNF-α mRNA/I-κBαKey words
polysaccharides from dendrobium officinale/ RAW264.7 cells/ cell proliferation/ TMF-α/ TMF-a mRMA/ l-κBα分类
医药卫生引用本文复制引用
蔡海兰,黄晓君,聂少平,田芳,谢明勇,崔武卫..铁皮石斛多糖对RAW2647细胞分泌TNF-α的影响[J].中国药理学通报,2012,28(11):1553-1556,4.基金项目
国家自然科学基金资助重点项目(No 31130041) （No 31130041）
食品科学与技术国家重点实验室目标导向项目(No SKLF-MB-201001) （No SKLF-MB-201001）
江西省青年科学家(井冈之星)培养对象资助项目 （井冈之星）
