中国药理学通报2012，Vol.28Issue(11)：1557-1562,6.DOI:10.3969/j.issn.1001-1978.2012.11.018
革皮氏海参总皂苷对小鼠骨髓细胞周期和细胞凋亡的影响
Effects of saponin of pearsonothuria graeffei on bone marrow cells in myelosuppression mice induced by cyclophosphamide
摘要
Abstract
Aim To investigate the effects of saponin of pearsonothuria graeffei ( SP )on coll cycle and apoptosis of hone marrow cells in cyclophosphamide( CY )-induced myelosuppression mice. Methods The myelosuppression mice model was generaLed after injected in-traperitoneally with CY( 100 mg · kg-1 ). The myelosuppression mice were divided into three groups: the model control group and saponin group treated with SP 10mg/( kg · d ) respectively. The other mice were given with 0. 5% CMC solution as placebo. All agents were administered once a day for 16 d. Changes in cell cycle and apoptosis of hone marow cells were detected with FCM ( flow cylometry ). The expressions of cy-clinBl ,bcl-2,bcl-xl were determined with reverse tran-scription PCR( RT-PCR ). Results Saponin of pear-sonothuria graeffei significantly elevated hone marrow nucleated cell( BMNC ) and promoted the cell cyclc from S phase to G2/M phase, the expression of cy-clinBl was also increased in saponin group. The apop-tosis rate in myelosuppression mice was significantly reduced and the expression levels of bo 1-2 and bcl-xl were olovatod in saponin group compared vvith the mod- el control mice. Conclusion Saponin of pearsonothu-ria graeffei is beneficial for improving bone marrow cells division and proliferation, inhibking cell apopto-sis, recovering the function of hematopoiesis.关键词
革皮氏海参总皂苷/骨髓抑制/流式细胞术/cyclinB1/bcl-2/bcl-xlKey words
saponin of pearsonothuria graeffei/ myelosuppression/ flow cylometry / cyclinBl / bo 1-2/ bcl-xl分类
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贺敏,王静凤,柳东,李冰,王玉明,薛长湖..革皮氏海参总皂苷对小鼠骨髓细胞周期和细胞凋亡的影响[J].中国药理学通报,2012,28(11):1557-1562,6.基金项目
国家自然科学基金资助项目海洋公益性行业科研专项(No 201105029,31000795) （No 201105029,31000795）
"泰山学者"建设工程专项经费 （）
