中国药理学通报2012，Vol.28Issue(11)：1570-1573,4.DOI:10.3969/j.issn.1001-1978.2012.11.021
原代培养的大鼠远端肺动脉平滑肌细胞的功能研究
Function of primary culture of rat distal pulmonary arterial smooth muscle cells
摘要
Abstract
Aim To investigate the function of primary culture of rat distal pulmonary arterial smooth muscle colls ( PASMC ) for in vitro studics of the pathophysiol-ogy and medicine intervcntion of pulmonary circulation diseases, including hypoxic pulmonary hypertension. Methods Microsurgery operations and collagenase di-gesLion were performed to isolate and cukure rat distal PASMC. 'Ihe rat distal PASMC were identified by morphological acLivity and immunofluorescence of smooth muscle a-acLin. Furthermore, the response of intracel-lular Ca2+ concentration ([ Ca2+ ]; ) of PASMC to 60 mmol o L-1 KC1 was tested by fluorescence microscope. Results The rat distal PASMC displayed a typical peak and vallcy growth pattern and smooth muscle a-actin showed a positive result. In addition, PASMC showed a clear increase in [ Ca2+ ]; in response to 60 mmol ·L-1 KC1 and nifedipine given at a 5 μmol o L-1 concentration completely blocked the [ Ca2+ ], response to KC1 in PASMC. Conclusion The rat distal PASMC show the morphological and im-munological characteristics of vascular smooth muscle cells and possess the voltage-dependent Ca2+ channels ( VDCC ) with normal functions. PASMC can be used to further explore the pathophysiological mechanisms and medicine intervention of pulmonary circulation diseases, including hypoxic pulmonary hypertension.关键词
肺动脉/平滑肌细胞/原代培养/大鼠/电压依赖性钙通道/肺动脉高压Key words
pulmonary arteries/ smooth muscle cells/ primary cukure/ rat/ the voltage-dependent Ca2+ channels / pulmonary hypertension分类
医药卫生引用本文复制引用
彭公永,周玉民,胡国平,蒋永亮,胡锦兴..原代培养的大鼠远端肺动脉平滑肌细胞的功能研究[J].中国药理学通报,2012,28(11):1570-1573,4.基金项目
国家自然科学基金项目资助(No 81000020) （No 81000020）
羊城学者科研计划学术骨干项目资助(No 10A025G) （No 10A025G）
广州市属高校科研项目资助(No 10A276) （No 10A276）
呼吸疾病国家重点实验室青年科学基金支持项目资助(No 11) （No 11）
