中国药理学与毒理学杂志2012，Vol.26Issue(5)：630-634,5.DOI:10.3867/j.issn.1000-3002.2012.05.006
去甲斑蝥素对体外微管蛋白聚合的抑制作用
Inhibitory effect of norcantharidin on tubulin polymerization in vitro
摘要
Abstract
OBJECTIVE To investigate the effect of norcantharidin ( NCTD) on the polymer-ization-depolymerization process of tubulin in vitro. METHODS Microtubule was isolated from porcine brain and identified by SDS-PAGE. The concentration of tubulin was determined by Bradford method. After NCTD at concentrations of 10, 20 and 30 μmol·L-1 was added into the tubulin polymerization-deporymerization system in vitro, the absorbance changes were measured under 350 nm at 37℃ and 4℃ respectively. Human ovarian tumor SK-OV-3 cells were used to analyze the effect of NCTD on tubulin polymerization-depolymerization at a cellular level. Microtubulin in supernatant and precipitation of SK-OV-3 cells was detected respectively by Western blotting after being treated by NCTD 60 μmol·L-1 for 8 h. RESULTS Tubulin polymerization was inhibited by NCTD in a concentration-dependent manner in 0~30 μmol·L-1 at 371. The inhibitory rate treated by different concentrations of NCTD in 10, 20 and 30 μmol·L-1 was (5. 5 ±2. 7)% , (7. 1 ±1.2)% and (27.5 ±0.4)%, respectively. However, the effect of NCTD on tubulin depolymerization at 4℃ was in significant. NCTD 60 μmol·L-1 increased free tubulin(91.5 ±8. 5)% and decreased combinative tubulin(51. 8 ±3. 8)% in human ovarian cancer SK-OV-3 cells, suggesting that NCTD can inhibit tubulin polymerization at a cellular level. CONCLUSION NCTD can inhibit tubulin polymerization in vitro.关键词
去甲斑蝥素/微管蛋白Key words
norcantharidin/tubulin分类
医药卫生引用本文复制引用
洪兴福,李宝赛,孙震晓..去甲斑蝥素对体外微管蛋白聚合的抑制作用[J].中国药理学与毒理学杂志,2012,26(5):630-634,5.基金项目
北京中医药大学自主选题项目资助(2009JYBZZ-JS038) （2009JYBZZ-JS038）
北京中医药大学自主选题项目资助(2011JYBZZ-XS037) （2011JYBZZ-XS037）
北京中医药大学自主选题项目资助(2011JYBZZ-XS054) （2011JYBZZ-XS054）
