中国油料作物学报2012，Vol.34Issue(4)：343-347,5.
大豆膨胀素GmEXPA4基因的克隆及表达分析
Cloning and expression of expansin GmEXPA4 gene in soybean
摘要
Abstract
Degenerate primers were designed according to expansin gene sequences from other plants. Conserved sequence of expansin gene in Glycine max L. was cloned using young shoot cDNA of a dwarf mutant HK808. Full-length expansin gene, designated as GmEXPA4, was cloned by RACE. Bioinformatics result showed that GmEXPA4 was a member of alpha-expansin subfamily. Fluorescence real-time quantification PCR indicated that GmEXPA4 expression was constitutively in different period in wildtype Dongnong 42 and mutant HK808. The relative expression showed close relation to the amount of soybean growth.关键词
大豆/矮化突变体/膨胀素/克隆/实时荧光定量/器官表达Key words
Glycine max L./Dwarf mutant/Expansin/Clone/Real-time quantification PCR/Expression in organs分类
农业科技引用本文复制引用
闻静,李凤兰,赵刚,孙丽,孙莉莉,胡宝忠..大豆膨胀素GmEXPA4基因的克隆及表达分析[J].中国油料作物学报,2012,34(4):343-347,5.基金项目
黑龙江省自然科学基金(230340) （230340）
黑龙江省教育厅科学技术研究项目(12511061) （12511061）
