中国中医药信息杂志2012，Vol.19Issue(10)：38-40,3.DOI:10.3969/j.issn.1005-5304.2012.10.015
抵挡汤早期干预对2型糖尿病大鼠肿瘤坏死因子-α与血管细胞黏附分子-1表达的影响
Effects of Early Intervention with Didang Decoction on Expression of VCAM-1 and TNF-α in Experimental Type 2 Diabetic Rats
摘要
Abstract
Objective To observe the protective effects of the early intervention with Didang decoction on the experimental type 2 diabetic rats with macrovascular lesions. Methods Experimental diabetic rats were induced by high fat diet and streptozotocin. The rats in Didang decoction late group, Didang decoction middle group and Didang decoction early group were given Didang decoction from 4 weeks before modeling, modeling completed and 4 weeks after modeling respectively to 24th week. The rats in simvastatin group and rosiglitazone group were given simvastatin and rosiglitazone respectively from modeling completed to 24th week. The level of VCAM-1 in serum was detected by ELASA and the mRNA expression of TNF-a in aorta using real-time PCR. Results Compared with normal control group, the level of VCAM-1 in serum and the mRNA expression of TNF-a in aorta were higher in model control group (P< 0.05). Compared with model control group, the level of VCAM-1 in serum was lower in Didang decoction early group and simvastatin group (P<0.05), the mRNA expression of TNF-a in aorta was lower in Didang decoction early group, Didang decoction middle group and rosiglitazone group (P<0.05). Conclusion The level of VCAM-1 in serum was reduced and the mRNA expression of TNF-a in aorta of experimental type 2 diabetic rats was inhibited after early intervened with Didang decoction. Thus the inflammatory injury of macrovascular lesions was inhibited, the development of diabetic vascular disease was delayed, and the great vessels was protected.关键词
抵挡汤/糖尿病/大血管病变/肿瘤坏死因子-α/血管细胞黏附分子-1/大鼠Key words
Didang decoction/ diabetes mellitus/ macrovascular disease/ TNF-α/ VCAM-1/ rats分类
医药卫生引用本文复制引用
常柏,李巧芬,常宝成,潘从清,孟东,陈莉明,李春深..抵挡汤早期干预对2型糖尿病大鼠肿瘤坏死因子-α与血管细胞黏附分子-1表达的影响[J].中国中医药信息杂志,2012,19(10):38-40,3.基金项目
国家自然科学基金(30973753) （30973753）
