中国中医药信息杂志2012，Vol.19Issue(10)：41-43,3.DOI:10.3969/j.issn.1005-5304.2012.10.016
HPLC同时测定陇中损伤胶囊中3种皂苷含量
Simultaneously Determination of Three Kinds of Ginsenosides in Longzhong Sunshang Capsule by HPLC
摘要
Abstract
Objective To establish the method for simultaneous determination of panax notoginseng saponins R1, ginseng saponins Rg1, ginseng saponins Rb1 in Longzhong Sunshang Capsule by HPLC with gradient elution. Methods C18 column (250 nm×4.6 nm, 5 μm) was used with mobile phase of acetonitrile (A)-0.05% phosphoric acid solution (B), gradient elution 0-32 min (A 23%-23%), 32-42 min (A 23%-30%), 42-67 min (A 30%-46%). The detection wavelength was 203 nm, and the flow rate was 1.0 mL/min. Results The linear relationship of panax notoginseng saponins Ri, ginseng saponins Rg1, ginseng saponins Rb1 was in the range of 1.02-8.50 μg (r=0.999 5), 0.90-7.50 μg (r =0.999 0) and 1.17-9.75 μg (r = 0.999 6) respectively. The average recovery was 100.98% (RSD = 2.71%), 97.73% (RSD = 1.98%) and 99.57% (RSD = 1.62%). Conclusion The method is simple, accurate, reliable, and can be used for quality control of Longzhong Sunshang Capsule.关键词
陇中损伤胶囊/三七皂苷R1/人参皂苷Rg14人参皂苷Rb1/含量测定Key words
Longzhong Sunshang Capsule/ panax notoginseng saponins R1/ Ginseng saponins Rg1/ Ginseng saponins Rb1/ content determination分类
医药卫生引用本文复制引用
王晓琳,张平,郭敏,李晓东,姜华..HPLC同时测定陇中损伤胶囊中3种皂苷含量[J].中国中医药信息杂志,2012,19(10):41-43,3.基金项目
兰州市科技局立项课题(2010-1-161) （2010-1-161）
