中国中医药信息杂志2012，Vol.19Issue(10)：58-60,3.DOI:10.3969/j.issn.1005-5304.2012.10.022
高效毛细管电泳法测定灯盏花素固体分散体中野黄芩苷含量
Determination of Scutellarin in Breviscapine Solid Dispersions by HPCE
摘要
Abstract
Objective To establish the method for determination of scutellarin in Breviscapine Solid Dispersions by HPCE. Methods The separation was performed on a fused silica capillary (60 cm×75 μm), with 40 mmol/L Na2B4O7 (25℃, pH 9.0) as the running buffers. The separation voltages were 20 kV, injection time was 5 s, temperature was 25℃, and the detection wavelength was 280 nm. Results The concentration range of scutellarin was 0.1-4 mg/mL with linear ralationship (r=0.999 2). The average recovery was 99.35% (RSD = 0.47%, n=9). Conclusion The method was stable and feasible for the determination of scutellarin in Breviscapine Solid Dispersions.关键词
灯盏花素固体分散体/野黄芩苷/高效毛细管电泳Key words
Breviscapine Solid Dispersions/ scutellarin/ HPCE分类
医药卫生引用本文复制引用
贾永艳,崔永霞,魏超娟,祝侠丽,黄海英,田效志..高效毛细管电泳法测定灯盏花素固体分散体中野黄芩苷含量[J].中国中医药信息杂志,2012,19(10):58-60,3.基金项目
河南省教育厅自然科学研究项目(2006350006) （2006350006）
