浙江大学学报（理学版）2012，Vol.39Issue(6)：689-695,7.DOI:10.3785/j.issn.1008-9497.2012.06.017
10种杓兰属植物rDNA ITS序列的克隆与分析
Cloning and analysis of rDNA ITS sequences from ten Cypripedium plants
蒋明 1李温平 1周晶 1陈贝贝1
作者信息
- 1. 台州学院生命科学学院,浙江临海317000
- 折叠
摘要
Abstract
Ten Cypripedium plants were used as materials for internal transcribed spacer (ITS) sequence cloning by PCR methods. Sequencing results revealed that the full length ITS sequences of Cypripedium ranged from 522 to 572 bp with 194 variable sites,99 parsimony-informative sites and 95 single nucletide polymorphism sites. All the 5. 8S sequences were 170 bp in length with rich variable sites including 30 parsimony-informative sites and 20 single nucletide polymorphism ones. The specific sites in ITS sequences could be used to identify these ten Cypripedium plants. Phylogenetic analysis results indicated that the plants studied constituted three groups: group 1 for C. henr-yi, C. flavum, C. tibeticum and C, guttatum, group 2 for C. himalaicum, C. macranthum, C. smithii and C. calceolus, and group 3 for C. margaritaceum and C. plectrochilum. In this study, ITS sequences of ten Cypripedium were cloned and analyzed providing the foundation for genetic diversity and phylogenetic analysis.
关键词
杓兰属/ITS序列/克隆/分子鉴定Key words
Cypripedium/ ITS sequence/ cloning/ molecular identification分类
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蒋明,李温平,周晶,陈贝贝..10种杓兰属植物rDNA ITS序列的克隆与分析[J].浙江大学学报（理学版）,2012,39(6):689-695,7.
