浙江农林大学学报2012，Vol.29Issue(5)：696-702,7.
美国松材线虫体表携带优势细菌的鉴定及致病性
Identification and pathogenicity of bacterial strains carried by American pine wood nematodes
摘要
Abstract
To confirm that pine wood nematodes carrying attenuated bacterial strains could alleviate pine wilt disease, bacteria were isolated from two nematodes native to America. Bacterial virulence and pathogenicity were tested with pine seedlings, and preliminary identification of predominant strains was determined using bacterial staining reactions and morphology combined with 16S rDNA sequence analysis. Results of the isolation experiment showed that the predominant bacteria which American nematodes carried were strains MG4 (Delftia tsuruhatensis), MG5 (Pseudomonas putida), MG8 (Stenotrophomonas maltophilia), and MG9 (Pan-toea sp.) Virulence and pathogenicity results demonstrated that compared with the high virulent bacteria strains (Pseudomonas fluorescence) isolated from Chinese nematodes, toxicity and pathogenicity of bacterial strains from America were relatively low. Thus, the bacteria carried by American nematodes should be considered the candidates for biological control agents.关键词
森林保护学/松材线虫/优势细菌/毒性/致病性/细菌鉴定Key words
forest protection/ Bursaphelenchus xylophilus / dominant bacteria/ toxicity/ pathogenicity/ identification分类
农业科技引用本文复制引用
曾腓力,贲爱玲,郑敬荣,韩正敏..美国松材线虫体表携带优势细菌的鉴定及致病性[J].浙江农林大学学报,2012,29(5):696-702,7.基金项目
国家自然科学基金资助项目(31170600) （31170600）
