浙江农林大学学报2012，Vol.29Issue(5)：790-794,5.
木瓜花粉生活力测定及储藏特性
Pollen viability and storage of Chaenomeles sinensis
摘要
Abstract
To determine the pollen viability of Chaenomeles sinensis, methods of culture medium, iodine/ potassium iodide solution (I-KI) staining and 1% 2,3,5-triphenyl tetrazolium chloride (TTC) staining were used to determine the pollen germination. Effects on pollen germination for different storage types and time were also studied. Results showed that the best culture medium was 100 mg·L-1 sucrose + 30 mg·L-1 boric acid + 8 g·L-1 agar, with the germination rate reaching 75.1%. For pollen viability, TTC staining with a germination rate of 60.6% was suitable; however, I-KI staining with germination rate of only 17.9% was not suitable. The best storage temperatures, in turn, were -80 ℃, 4 ℃, -23 ℃ and room temperature. At -80 ℃, the germination rate of pollen was 22.9% when it was preserved for 16 days.关键词
植物学/木瓜/花粉/生活力/储藏条件Key words
botany/ Chaenomeles sinensis/ pollen/ viability/ storage conditions分类
农业科技引用本文复制引用
管雨,贾文庆,刘会超,张智俊..木瓜花粉生活力测定及储藏特性[J].浙江农林大学学报,2012,29(5):790-794,5.基金项目
浙江省自然科学基金资助项目(Y307469) （Y307469）
浙江省现代森林培育技术重点实验室开放基金资助项目(200605) （200605）
