中山大学学报（医学科学版）2012，Vol.33Issue(5)：630-633,4.
结直肠癌组织E-钙黏附素蛋白表达与甲基化的关系
Relationship and Significance of Expression of E-cadherin Proteins and Methylation of Promoter in Colorectal Cancer Tissues
摘要
Abstract
[Objective] To explore the relationship between methylation of promoter and the expression of E-cadherin proteins in colorectal cancer. [Methods] Streptavidin-peroxidase (S-P) two-step method was used to measure the immunohistochemical expression of E-cadherin proteins in 100 CRC. Those slides exhibiting diffuse immunostaining presenting more than 75% of tumor cells were classified as (+++), between 50% and 75% were classified as (++), between 25% and 50% were classified as ( + ) , and those with immunoreactivity less than 10% were classified as (-).(-) and (+) were combine together as lower expression cases, while (++) and (+++) were combined together as higher expression cases for statistical analysis. [Results] The positive rate of E-cadherin proteins was 76% (+~+++) in 100 sporadic colorectal cancer specimen. Fifty-nine cases showed higher expression of E-cadherin, while decreased E-cadherin immunohistochemical expression was observed in 41 cases. The methylation positive rate was 68.3% of E-cadherin was significant association with protein decreased expression of E-cadherin gene (P < 0.001). [Conclusion] Methylation of E-cadherin gene was significant association with E-cadherin protein decreased expression. The loss of E-cadherin protein was one of the important influence factors in colorectal cancer metastatic and invasive.关键词
E-钙黏附素/免疫组化和甲基化/结直肠癌Key words
E-cadherin/ immunohistochemical and methylation/ colorectal cancer分类
医药卫生引用本文复制引用
黄美近,彭慧,杨祖立,康亮,王磊,王辉,刘焕亮,汪建平..结直肠癌组织E-钙黏附素蛋白表达与甲基化的关系[J].中山大学学报（医学科学版）,2012,33(5):630-633,4.基金项目
广东省科技计划基金(2011B080701064) （2011B080701064）
