作物学报2012，Vol.38Issue(9)：1561-1569,9.DOI:10.3724/SP.J.1006.2012.01561
拟南芥RBCS-1A基因受光调节表达模式及其启动子遗传转化应用评价
Expression Patterns of Arabidopsis RBCS-1A Gene in Response to Light Treatments and Application Evaluation of Its Promoter in Transgenic Engineering
摘要
Abstract
RBCS gene encodes the small subunit of Ribulose-l,5-bisphosphate carboxylase/oxygenase (Rubisco), which catalyzes the rate-limiting step of CO2 fixation in photosynthesis. In this study, we analyzed the transcription patterns of Arabidopsis RBCS-1A in response to light treatments using real-time quantitative PCR assays. The results indicated that the transcription abundance of AtRBCS-lA was induced by light, and had the tissue-specific feature. In silico analysis revealed that the promoter region of AtRBCS-1A keeps many cis-acting elements for light regulation. Based on the information, we isolated a genomic fragment with 1 691 nucleotides upstream of the translation start codon of AtRBCS-1Aand constructed a binary vector with AtRBCS-lA promoter fused with GUS (encoding p-glucuronidase) reporter gene, then generated Arabidopsis transgenicplants. Histochemical analysis of the transgenic lines suggested that AtRBCS-lA promoter was a light-induced and tissue-specific promoter. All the analyses preliminarily prove that AtRBCS-lA promoter has important application value in plant transgenic engineering.关键词
拟南芥/RBCS-1A基因/表达模式/光诱导表达/顺式作用元件Key words
Arabidopsis/RBCS-1A gene/Expression pattern/Light-induced expression/cis-acting elements引用本文复制引用
习雨琳,周朋,宋梅芳,李志勇,孟凡华,杨建平..拟南芥RBCS-1A基因受光调节表达模式及其启动子遗传转化应用评价[J].作物学报,2012,38(9):1561-1569,9.基金项目
本研究由国家转基因生物新品种培育重大专项(2011ZX08010-002)和重庆市自然科学基金(CSTC2009BA1088)项目资助. （2011ZX08010-002）
