作物学报2012,Vol.38Issue(9):1570-1582,13.DOI:10.3724/SP.J.1006.2012.01570
大豆NF-YB家族全基因组鉴定、分类和表达
Genome-Wide Identification, Classification, and Expression of NF-YB Gene Family in Soybean
摘要
Abstract
Based on soybean genome database and bioinformatics method, we obtained soybean NF-YB family genes and their positions on chromosome and duplication information. NF-YB proteins were classified according to their phylogenetic relationship. The soybean RNA-sequencing data from the SoyBase database and EST data from NCBI UniGene were used to analyze the expression pattern of these genes at different development stages. A total of 28 NF-YB genes were systematically identified from soybean and classified into three types. They were located on 14 chromosomes and most of them had stress related cis-acting elements in their promoter regions. Ten differentially expressed genes were found at each developmental stage, and four of them were highly expressed in different tissues other than root nodules and root. Among other six genes, two were highly expressed in root nodule, two were specifically expressed in root nodules and two were specifically expressed in root. The results facilitate functional analysis and utilization of NF-YB genes in crop genetic improvement.关键词
NF-YB家族/全基因组鉴定/进化/差异表达/启动子Key words
NF-YB/Genome-wide identification/Phylogeny/Differential expression/Promoter引用本文复制引用
郑炜君,徐兆师,冯志娟,李连城,陈明,柴守诚,马有志..大豆NF-YB家族全基因组鉴定、分类和表达[J].作物学报,2012,38(9):1570-1582,13.基金项目
本研究由国家自然科学基金项目(31171546)和国家转基因生物新品种培育科技重大专项(2011ZX08002-002,2009ZX08009-083B)资助. (31171546)