作物学报2012，Vol.38Issue(9)：1649-1656,8.DOI:10.3724/SP.J.1006.2012.01649
棉花S-腺苷甲硫氨酸脱羧酶基因的克隆及低温下的表达分析
Molecular Cloning and Expression Analysis of GhSAMDC at Low Temperature Stress in Cotton (Gossypium hirsutum L.)
摘要
Abstract
S-adenosylmethionine decarboxylase is one of the key enzymes in polyamine biosynthesis. With the increase of poly-amine accumulation in plants, SAMDC can improve crop tolerance to environment stresses. We cloned the S-adenosylmethionine decarboxylase gene from Gossypium hirsutum by RACE and RT-PCR, named GhSAMDC (GenBank accession number: JN020148). The cDNA full length was 1 874 bp, with three open reading frames, i.e. tiny ORF (tORF), upstream ORF (uORF) and main ORF (mORF). The mORF was 1 064 bp encoding 355 amino acids with a molecular mass of 38.25 kD, and its deduced protein had two highly conserved function domains (proenzyme cleavage site and PEST domain). The complete genomic DNA sequence of GhSAMDC was 2 743 bp in length with three introns, all of which were located in 5' UTR, and one lay in the uORF. Phylogenetic analysis showed GhSAMDC was the closest to that in Vitis vinifera, and real-time PCR suggested that GhSAMDC was induced by cold stress. It showed lower gene expression level in cold resistant cultivar Xinluzao 33 than in cold sensitive cultivar Xinluzao 1.关键词
棉花/S-腺苷甲硫氨酸脱羧酶/序列分析/定量PCR/低温表达Key words
Cotton (Gossypium hirsutum L.)/S-adenosylmethionine decarboxylase/Sequence analysis/qPCR/Low temperature引用本文复制引用
耿卫东,李艳军,张新宇,朱华国,孙杰..棉花S-腺苷甲硫氨酸脱羧酶基因的克隆及低温下的表达分析[J].作物学报,2012,38(9):1649-1656,8.基金项目
本研究由兵团博士资金项目(2011BB004)和国家转基因生物新品种培育重大专项子课题(2008ZX08005-005)资助. （2011BB004）
