植物研究2012，Vol.32Issue(4)：444-451,8.
杜仲1-羟基-2-甲基-2-(E)-丁烯基-4-二磷酸合酶基因cDNA全长克隆与序列分析
Cloning and Sequence Analysis of 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate Synthase Gene cDNA from Eucommia ulmoides
摘要
Abstract
l-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase (HDS) catalyses 2C-methyl-D-erythritol-2,4-cyclodiphosphate into l-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate in the penultimate step of 2C-meth-yl-D-erythritoI 4-phosphate (MEP) pathway, which is an alternative plant terpenoids biosynthetic route that has been recently discovered. Homologous HDS cDNA (EuHDS) was isolated from leaves of Eucommia ulmoides by the methods of reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends ( RACE ) technique. The full-length cDNA of EuHDS was 2 786 bp and encoded 743 amino acids with a predicted molecular mass of 82. 25 kD, and a theoretical isoelectric point of 5. 89. Two conserved motifs and three absolutely conserved cysteines (residues 644, 647 and 678) were discovered in the deduced coding sequence. Phylogenetic analysis revealed that EuDXR was more similar to Vitis vinifera(evolutionary distance, 0.049) than that of other species, followed by Solanum lycopersicum(0.052) and Hevea brasiliensis(0. 052).关键词
杜仲/HDS/基因/序列分析Key words
Eucommia ulmoides/HDS/gene/sequence analysis分类
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刘攀峰,杜红岩,杜兰英,乌云塔娜,黄海燕..杜仲1-羟基-2-甲基-2-(E)-丁烯基-4-二磷酸合酶基因cDNA全长克隆与序列分析[J].植物研究,2012,32(4):444-451,8.基金项目
国家公益性行业科研专项(201004029) （201004029）
