植物研究2012，Vol.32Issue(4)：452-457,6.
山麦冬及其近缘种DNA条形码研究
Analysis of Liriope spicata and Its Related Species Based on DNA Barcoding Technique
摘要
Abstract
To discuss the potential of cpDNA trnL-F and psbA-trnH as DNA barcode of Liriope spicata ( Thun-berg) Loureiro and its related species, cpDNA trnL-F and psbA-trntl sequences were analysed. These two regions were respectively amplified by PCR and then purified and sequenced successfully. The results showed that the lengths of trnL-F sequences in 18 plant samples belonging to 9 species of liriopogons varied from 355 to 356 bp. There was no difference in the trnL-F of various species of liriope, but some specifically variable sites were found in this fragment, which led to the identification between Ophiopogon japonicus ( Thunb. ) Ker-Gawl and Ophiopogon bodinieri Levl. The length of psbA-trnH region was 543 -544 bp, there were only some identification sites in 0. japonicus (Thunb. ) Ker-Gawl. Because of the low rate of evolution and the high conservation, these two cpDNA spacer regions could be only appropriate as a DNA barcode to identify liriopogons at generic level, but there is a degree of limit at lower taxonomic level.关键词
山麦冬属/沿阶草属/trnL-F序列/psbA-trnH序列Key words
Liriope/Ophiopogon/trnL-F/psbA-trnH分类
农业科技引用本文复制引用
许金榜,张君毅..山麦冬及其近缘种DNA条形码研究[J].植物研究,2012,32(4):452-457,6.基金项目
福建省农业科技重点项目(2009N0041) （2009N0041）
中央高校基本科研业务(JB-ZR1115) （JB-ZR1115）
