植物研究2012，Vol.32Issue(5)：591-595,5.
千里光肌动蛋白基因(Actin)的生物信息学及功能分析
Functional Analysis of Actin Gene from Senecio scandens Buch.-Ham.ex D.Do Based on Its Bioinformatics
摘要
Abstract
Actin, a constitutively expressed protein in higher plants, was closely related to the function such as cell division, cell movement and cell signal transduction. In order to learn the relationship between characteristics and functional roles, actin genes from full-length cDNA library in Senecio scandens Buch. -Ham. ex D. were isolated, and it was found to share 96% indentity with Glycyrrhiza uralensis ( GenBank accession; ABW71681. 1) using bioinformatics analysis. In the present study, actin protein was composed of 360 amino acid residues with the predicted molecular weight of 40. 02 kDa and the theoretical isoelectric point of 5. 85. Analyses of domain and structure indicated that the protein was dominantly composed of the imperative curls and the loosed helixes, with 4 domains in three-dimensional level. The phylogenetic tree of 28 species suggested that closer relationship was observed among S. scandens, Cicer arietinum, and G. uralensis. Notably, it was concluded that actin protein might play an important role in the regulation of gene transcription in higher plants, without changeable advanced structure even if a few amino acid mutation sites.关键词
千里光/Actin基因/序列分析/结构预测Key words
Senecio scandens Buch. -Ham. ex D. Do/Actin gene/sequence analysis/structure prediction分类
农业科技引用本文复制引用
张珍,平军娇,蔡振锋,汤贤春,钱刚..千里光肌动蛋白基因(Actin)的生物信息学及功能分析[J].植物研究,2012,32(5):591-595,5.基金项目
贵州省优秀科技教育人才省长基金项目(黔省专合字2008-61号) （黔省专合字2008-61号）
