中国中西医结合杂志2012，Vol.32Issue(10)：1331-1335,5.
慢性胃炎患者腻苔的口腔微生物指纹图谱分析
The Oral Microbial Fingerprint on the Greasy Tongue Coating of Patients with Chronic Gastritis
摘要
Abstract
Objective To investigate the microbial changes on the greasy tongue coating of the patients with chronic gastritis and to explore the formation mechanism of greasy tongue coating. Methods Forty cases of tongue coating samples from patients with chronic gastritis were collected, 20 cases of greasy fur (as the greasy fur group), 20 cases of non-greasy fur (as the non-greasy fur group), and 20 cases of tongue coating samples from healthy subjects (as the healthy control group). Using 16S rRNA gene denatured gradient gel electrophore-sis (DGGE) the microbial population of the tongue coating was detected. The DGGE fingerprint of the bacterium on the tongue coating was obtained. After digitalized principle component analysis ( PCA) and partial least squares (PLS-DA) were performed. Results The microorganism compositions are different in the greasy fur group, the non-greasy fur group, and the healthy control group. (1) There were five significantly different bands between the greasy fur group and the non-greasy fur group, with the accuracy of 97.5% in judging the model. There were 8 significantly different bands between the greasy fur group and the healthy control group, with the ac-curacy of 95.0% in judging the model. There was no obvious difference between the healthy control group and the non-greasy fur group. (2) The brightness of band 8 was higher in the greasy fur group than in the non-greasy fur group and the healthy control group. It may be a new species closely associated with the formation of greasy tongue coating. Results of the sequence showed its nearest neighbor was Moraxella catarrhalis, but with the simi-larity of 96.2%. The brightness of band 10 was sequenced as the healthy control group >the non-greasy fur group >the greasy fur group. Results of the sequence showed it had 100. 0% similarity to Rothia mucilaginosa (stick-slip Ross strain). Conclusions The bacteria species on band 8 may have a close correlation with the for-mation of greasy fur of chronic gastritis, while the bacteria species on band 10 may have a close correlation with the formation of non-greasy fur. They indicated the microbial changes in the oral cavity may be one of the forma-tion mechanisms for greasy tongue coating.关键词
慢性胃炎/舌诊/腻苔/微生态学变性梯度凝胶电泳方法Key words
chronic gastritis/ tongue diagnosis/ the greasy tongue coating/ micro-ecology denatured gradient gel electrophoresis method引用本文复制引用
李福凤,赵洁,庞小燕,钱鹏,陈慧,王忆勤,叶进,付晶晶,孙祝美..慢性胃炎患者腻苔的口腔微生物指纹图谱分析[J].中国中西医结合杂志,2012,32(10):1331-1335,5.基金项目
上海高校选拔培养优秀青年教师科研专项基金资助项目(No.szy08001) （No.szy08001）
上海市重点学科(第三期)中医诊断学资助项目(No.S30302) （第三期）
上海中医药大学杏林学者基金资助项目(No.R09205) （No.R09205）
