中国中西医结合杂志2012，Vol.32Issue(10)：1385-1389,5.
草麻黄补体抑制成分对大鼠脊髓损伤后免疫炎症反应的影响
Effects of Complement Inhibiting Component of Ephedra sinica on Immunological Inflammation following Acute Spinal Cord Injury in Rats
摘要
Abstract
Objective To investigate the effects of complement inhibiting component of Ephedra sinica on immunological inflammation following acute spinal cord injury (SCI) in rats. Methods The complement inhibiting component of Ephedra sinica was isolated by multiple precipitation steps and thin layer chromatography, and then the activity was analyzed. Fifty healthy SD rats were selected and randomly divided into the control group and the experimental group, 25 in each group. Induction of SCI was performed following a modified Allen's weight-drop method. The complement inhibiting component from Ephedra sinica (15 mg/kg) dissolving in 5 mL normal saline was immediately administered by gastrogavage after SCI, once daily. Equal volume of normal saline was admin-istered to rats in the control group by gastrogavage. Hematoxylin and eosin (H&E) staining and C3 immunohisto-chemical staining were performed in SCI tissue at 12 h, day 1,3,7, and 14 after SCI. C3 positive expressions and myeloperoxidase (MPO) activity were assessed. Intercellular adhesion molecule-1 (ICAM-1) mRNA ex-pression level was evaluated by Real-time PCR technique. Results C3 positive expression, MPO activity, and ICAM-1 mRNA level were significantly weaker in the Ephedra sinica group than in the control group at all time points (12 h, day 1, day 3, day 7, and day 14 after SCI) ( P < 0. 01 , P < 0. 05). Conclusions There existed complement system activation following acute SCI. The complement inhibiting component of Ephedra sinica signif-icantly reduced immunological inflammation after SCI, and played an important role in secondary SCI.关键词
草麻黄/脊髓损伤/补体激活/炎症Key words
Ephedra sinica/ spinal cord injury/ complement activation/ inflammation引用本文复制引用
李良满,朱悦..草麻黄补体抑制成分对大鼠脊髓损伤后免疫炎症反应的影响[J].中国中西医结合杂志,2012,32(10):1385-1389,5.基金项目
辽宁省科学技术计划项目(No.2010225034) （No.2010225034）
