中国中西医结合杂志2012，Vol.32Issue(10)：1390-1393,4.
氧化苦参碱对人瘢痕成纤维细胞前胶原及纤维粘连蛋白表达的影响
Effects of Oxymatrine on the Expressions of Pro-collagen and Fibronectin of Fibroblasts Derived from Human Hyperplastic Scars
摘要
Abstract
Objective To study the effects of oxymatrine (OM) on the expressions of pro-collagen I (PC I ), pro-collagen 1 (PCffi), fibronectin (FN), matrix metalloproteinase-1 (MMP-1) mRNA of fibroblasts from keloid (KFb), hyperplastic scar (HFb), and normal skin (NFb), and to compare with hydrocortisone (HC). Methods The primary KFb, HFb and NFb were derived from patients and cultured in vitro using tissue block culture method. The fibroblasts were treated with 500 μg/mL OM, 2μg/mL HC, or without any medicine (as the control). The mRNA expressions of PC I , PCM , FN, MMP-1 of the fibroblasts were detected using RT-PCR. Results Under the normal condition, when compared with NFb, the mRNA expressions of PC I of KFb and HFb increased by 31. 7% and 34. 2% (both P<0. 05). Besides, the mRNA expression of PCI of KFb in-creased by 44. 9% (P<0. 01). OM down-regulated the mRNA expressions of FN and PC I of HFb by 18. 8% and 23. 6% respectively (both P < 0.05). HC decreased the mRNA expressions of FN and PC I of HFb by 26.8% and 43. 6% respectively (P<0. 05, P <0. 01). Meantime, OM up-regulated the mRNA expression of MMP-1 of KFb by 21. 8% (P <0. 05). Conclusions OM suppressed the synthesis of extracellular matrix (ECM) possibly through down-regulating the mRNA expressions of PC I and FN. Compared with HC, OM could pro-mote the degradation of ECM through inducing the MMP-1 mRNA expressions of KFb. Therefore, OM could be potentially used in treatment of hypertrophic scar and keloid.关键词
氧化苦参碱/成纤维细胞/细胞外基质/基质金属蛋白酶/增生性瘢痕/瘢痕疙瘩Key words
oxymatrine/ fibroblast/ extracellular matrix/ matrix metallo proteinase/ hyperplastic scar/ keloid引用本文复制引用
边曦,吴江群,聂兴举,秦泽莲..氧化苦参碱对人瘢痕成纤维细胞前胶原及纤维粘连蛋白表达的影响[J].中国中西医结合杂志,2012,32(10):1390-1393,4.基金项目
国家自然科学基金资助项目(No.30170971) （No.30170971）
